| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Conley, Davion L (18)
|
OZTCAY |
24.28 |
23.97 |
-0.31 |
| 2 |
Gutierrez, Sebastian V (18)
|
AZSAC |
24.90 |
24.17 |
-0.73 |
| 3 |
Tretyakov, Andrey D (16)
|
SEMSA |
25.00 |
24.41 |
-0.59 |
| 4 |
Eruchalu, Ikenna N (16)
|
NCMAC |
24.94 |
24.52 |
-0.42 |
| 5 |
Portmann, Jack R (17)
|
MADST |
24.95 |
24.65 |
-0.30 |
| 6 |
Iverson, Ivar M (17)
|
WISEA |
25.31 |
24.74 |
-0.57 |
| 7 |
Boyd, Dylan J (16)
|
ILUN-0 |
25.56 |
24.80 |
-0.76 |
| 8 |
Amdor, Wyatt I (17)
|
MAUDAC |
24.82 |
24.87 |
0.05 |
| 9 |
Winkler, John M (16)
|
STLCA |
25.19 |
25.17 |
-0.02 |
| 10 |
Grissom, Kyle A (17)
|
CCTNT |
24.06 |
25.38 |
1.32 |
| 11 |
Butler, Christopher W (17)
|
SEBAY |
26.08 |
25.69 |
-0.39 |